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multiplex pcr kit  (Qiagen)


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    Structured Review

    Qiagen multiplex pcr kit
    Multiplex Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 5795 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multiplex+pcr+kit/QIAGEN+Multiplex+PCR+Kit/10__3892_slash_br__2026__2158-53-7-10
    Average 99 stars, based on 5795 article reviews
    multiplex pcr kit - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Multiplex Assay:

    Article Title: Characterization of Antimicrobial Resistance and Potential Zoonotic Risk in Uropathogenic Escherichia coli Isolated from Companion Animals, with Genomic Analysis of Virulence Determinants in a Representative Isolate
    Article Snippet: .. Four primer sets ( ) targeting these genes, which have been previously used to assess the urovirulence potential of human UPEC, were combined in a single reaction for multiplex PCR using the QIAGEN ® Multiplex PCR Kit (Qiagen, Germantown, MD, USA). .. Each 25 μL PCR reaction mixture contained 12.5 μL of 2× QIAGEN Multiplex PCR Master Mix, 0.1 μM each of forward and reverse primers for the four target genes, and 20 ng of template DNA.

    Article Title: A synonymous SLC2A1 variant causes familial epilepsy and paroxysmal exercise-induced dyskinesia by creating aberrant mosaic splicing patterns.
    Article Snippet: .. The genomic region encompassing SLC2A1 exons 7 & 8 (with flanking introns) was PCR amplified with the Multiplex PCR Kit (QIAGEN, Cat: 206143) and purified with the QIAquick PCR Purification kit (QIAGEN, Cat: 28106), according to the manufacturer's protocols. ..

    Article Title: Characterization of Antimicrobial Resistance and Potential Zoonotic Risk in Uropathogenic Escherichia coli Isolated from Companion Animals, with Genomic Analysis of Virulence Determinants in a Representative Isolate
    Article Snippet: .. Four primer pairs ( ) targeting the genes chuA , yjaA , and arpA , and the DNA region TspE4.C2 were used in a single multiplex reaction with the QIAGEN ® Multiplex PCR Kit (Qiagen). .. Each 25 μL PCR mixture contained 12.5 μL of 2× QIAGEN Multiplex PCR Master Mix, 0.1 μM each of forward and reverse primers for the chuA , yjaA , TspE4.C2, 0.2 μM of arpA primer set and 20 ng of template DNA.

    Article Title: Long-term refined genomic analysis of tuberculosis clusters to distinguish between ongoing transmission, reactivations or diagnostic delays, Almería, Spain, 2003 to 2024
    Article Snippet: .. The standard protocol was followed using the multiplex PCR kit (Qiagen) for mixes 1, 2, 3, 5, 6 and 8 [ ]. .. For mixes 4 and 7, PuReTaq Ready-To-Go PCR beads (GE Healthcare, Chicago, United States (US)) were used with 0.5 mM of each primer and 3% dimethyl sulfoxide.

    Article Title: Human broadly neutralizing antibodies against the membrane-proximal external region of HIV Env for vaccine design and intervention
    Article Snippet: The first-strand cDNA from B cells was synthesized using Superscript III Reverse Transcriptase (Life Technologies) and random hexamers (Gene Link). .. Nested PCR amplification of HC and LC variable regions was performed using Multiplex PCR Kit (Qiagen). ..

    Article Title: P2X4 Drives Sex-Specific neuroprotection in autoimmune neuroinflammation.
    Article Snippet: The RNA was quantified via Qubit RNA BR or RNA HS Assays (Invitrogen). .. A total of 50 ng of RNA was used to synthesize cDNA with the NZY First-Strand cDNA Synthesis Flexible Pack (NZYTECH) and subsequently preamplified via the Multiplex PCR Kit (Qiagen). mRNA expression was analysed via the BioMark HD Nanofluidic qPCR System (Fluidigm) combined with 48.48 Dynamic Arrays IFC (Integrated Fluidic Circuits, IFC). ..

    Polymerase Chain Reaction:

    Article Title: Characterization of Antimicrobial Resistance and Potential Zoonotic Risk in Uropathogenic Escherichia coli Isolated from Companion Animals, with Genomic Analysis of Virulence Determinants in a Representative Isolate
    Article Snippet: .. Four primer sets ( ) targeting these genes, which have been previously used to assess the urovirulence potential of human UPEC, were combined in a single reaction for multiplex PCR using the QIAGEN ® Multiplex PCR Kit (Qiagen, Germantown, MD, USA). .. Each 25 μL PCR reaction mixture contained 12.5 μL of 2× QIAGEN Multiplex PCR Master Mix, 0.1 μM each of forward and reverse primers for the four target genes, and 20 ng of template DNA.

    Article Title: A synonymous SLC2A1 variant causes familial epilepsy and paroxysmal exercise-induced dyskinesia by creating aberrant mosaic splicing patterns.
    Article Snippet: .. The genomic region encompassing SLC2A1 exons 7 & 8 (with flanking introns) was PCR amplified with the Multiplex PCR Kit (QIAGEN, Cat: 206143) and purified with the QIAquick PCR Purification kit (QIAGEN, Cat: 28106), according to the manufacturer's protocols. ..

    Article Title: Characterization of Antimicrobial Resistance and Potential Zoonotic Risk in Uropathogenic Escherichia coli Isolated from Companion Animals, with Genomic Analysis of Virulence Determinants in a Representative Isolate
    Article Snippet: .. Four primer pairs ( ) targeting the genes chuA , yjaA , and arpA , and the DNA region TspE4.C2 were used in a single multiplex reaction with the QIAGEN ® Multiplex PCR Kit (Qiagen). .. Each 25 μL PCR mixture contained 12.5 μL of 2× QIAGEN Multiplex PCR Master Mix, 0.1 μM each of forward and reverse primers for the chuA , yjaA , TspE4.C2, 0.2 μM of arpA primer set and 20 ng of template DNA.

    Article Title: Long-term refined genomic analysis of tuberculosis clusters to distinguish between ongoing transmission, reactivations or diagnostic delays, Almería, Spain, 2003 to 2024
    Article Snippet: .. The standard protocol was followed using the multiplex PCR kit (Qiagen) for mixes 1, 2, 3, 5, 6 and 8 [ ]. .. For mixes 4 and 7, PuReTaq Ready-To-Go PCR beads (GE Healthcare, Chicago, United States (US)) were used with 0.5 mM of each primer and 3% dimethyl sulfoxide.

    Article Title: Human broadly neutralizing antibodies against the membrane-proximal external region of HIV Env for vaccine design and intervention
    Article Snippet: The first-strand cDNA from B cells was synthesized using Superscript III Reverse Transcriptase (Life Technologies) and random hexamers (Gene Link). .. Nested PCR amplification of HC and LC variable regions was performed using Multiplex PCR Kit (Qiagen). ..

    Article Title: P2X4 Drives Sex-Specific neuroprotection in autoimmune neuroinflammation.
    Article Snippet: The RNA was quantified via Qubit RNA BR or RNA HS Assays (Invitrogen). .. A total of 50 ng of RNA was used to synthesize cDNA with the NZY First-Strand cDNA Synthesis Flexible Pack (NZYTECH) and subsequently preamplified via the Multiplex PCR Kit (Qiagen). mRNA expression was analysed via the BioMark HD Nanofluidic qPCR System (Fluidigm) combined with 48.48 Dynamic Arrays IFC (Integrated Fluidic Circuits, IFC). ..

    Amplification:

    Article Title: A synonymous SLC2A1 variant causes familial epilepsy and paroxysmal exercise-induced dyskinesia by creating aberrant mosaic splicing patterns.
    Article Snippet: .. The genomic region encompassing SLC2A1 exons 7 & 8 (with flanking introns) was PCR amplified with the Multiplex PCR Kit (QIAGEN, Cat: 206143) and purified with the QIAquick PCR Purification kit (QIAGEN, Cat: 28106), according to the manufacturer's protocols. ..

    Article Title: Human broadly neutralizing antibodies against the membrane-proximal external region of HIV Env for vaccine design and intervention
    Article Snippet: The first-strand cDNA from B cells was synthesized using Superscript III Reverse Transcriptase (Life Technologies) and random hexamers (Gene Link). .. Nested PCR amplification of HC and LC variable regions was performed using Multiplex PCR Kit (Qiagen). ..

    Purification:

    Article Title: A synonymous SLC2A1 variant causes familial epilepsy and paroxysmal exercise-induced dyskinesia by creating aberrant mosaic splicing patterns.
    Article Snippet: .. The genomic region encompassing SLC2A1 exons 7 & 8 (with flanking introns) was PCR amplified with the Multiplex PCR Kit (QIAGEN, Cat: 206143) and purified with the QIAquick PCR Purification kit (QIAGEN, Cat: 28106), according to the manufacturer's protocols. ..

    Nested PCR:

    Article Title: Human broadly neutralizing antibodies against the membrane-proximal external region of HIV Env for vaccine design and intervention
    Article Snippet: The first-strand cDNA from B cells was synthesized using Superscript III Reverse Transcriptase (Life Technologies) and random hexamers (Gene Link). .. Nested PCR amplification of HC and LC variable regions was performed using Multiplex PCR Kit (Qiagen). ..

    cDNA Synthesis:

    Article Title: P2X4 Drives Sex-Specific neuroprotection in autoimmune neuroinflammation.
    Article Snippet: The RNA was quantified via Qubit RNA BR or RNA HS Assays (Invitrogen). .. A total of 50 ng of RNA was used to synthesize cDNA with the NZY First-Strand cDNA Synthesis Flexible Pack (NZYTECH) and subsequently preamplified via the Multiplex PCR Kit (Qiagen). mRNA expression was analysed via the BioMark HD Nanofluidic qPCR System (Fluidigm) combined with 48.48 Dynamic Arrays IFC (Integrated Fluidic Circuits, IFC). ..

    Expressing:

    Article Title: P2X4 Drives Sex-Specific neuroprotection in autoimmune neuroinflammation.
    Article Snippet: The RNA was quantified via Qubit RNA BR or RNA HS Assays (Invitrogen). .. A total of 50 ng of RNA was used to synthesize cDNA with the NZY First-Strand cDNA Synthesis Flexible Pack (NZYTECH) and subsequently preamplified via the Multiplex PCR Kit (Qiagen). mRNA expression was analysed via the BioMark HD Nanofluidic qPCR System (Fluidigm) combined with 48.48 Dynamic Arrays IFC (Integrated Fluidic Circuits, IFC). ..

    Real-time Polymerase Chain Reaction:

    Article Title: P2X4 Drives Sex-Specific neuroprotection in autoimmune neuroinflammation.
    Article Snippet: The RNA was quantified via Qubit RNA BR or RNA HS Assays (Invitrogen). .. A total of 50 ng of RNA was used to synthesize cDNA with the NZY First-Strand cDNA Synthesis Flexible Pack (NZYTECH) and subsequently preamplified via the Multiplex PCR Kit (Qiagen). mRNA expression was analysed via the BioMark HD Nanofluidic qPCR System (Fluidigm) combined with 48.48 Dynamic Arrays IFC (Integrated Fluidic Circuits, IFC). ..



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